Fsc-a New! Jun 2026

For particles smaller than the wavelength of the laser (e.g., extracellular vesicles, platelets), FSC-A becomes unreliable due to the dominance of Mie scattering and background noise. In these cases, Side Scatter (SSC) is often preferred for detection, or a dedicated small-particle detector is required.

: The intensity of the FSC signal is generally proportional to the diameter of the cell. Larger cells, such as monocytes, produce higher FSC signals than smaller cells, like lymphocytes. For particles smaller than the wavelength of the laser (e

Run a mix of small (3µm) and large (6-10µm) beads to check the dynamic range. Adjust FSC voltage so both populations are on scale (usually between 10^2 and 10^5 on a log scale or 100-200K on a linear scale). such as monocytes