Pharmacology begins long before synthesis. Using knowledge of disease pathology, pharmacologists identify biological targets—usually proteins, receptors, enzymes, or ion channels—that are implicated in a disease state. For example, in hypertension, the angiotensin-converting enzyme (ACE) is a validated target. However, a target is just a theory until validated. Pharmacologists use techniques like CRISPR gene editing or antisense oligonucleotides to "turn off" the target. If turning off the target alleviates the disease phenotype in cell cultures or animal models, the target is "validated."
Often described as "what the body does to the drug." It tracks the drug's journey through bsorption into the bloodstream, istribution to tissues, etabolism (often in the liver), and xcretion from the body. Pharmacodynamics (PD): pharmacology in drug discovery and development